Substrate Specificity

Engineering Novel Complement Activity into a Pulmonary Surfactant Protein

Complement activation / Biological Chemistry / Protein Engineering / Biological Sciences / Animals / Biological / Surface plasmon resonance / CHEMICAL SCIENCES / Rats / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Pulmonary Surfactant / Molecular Sequence Data / Biological / Surface plasmon resonance / CHEMICAL SCIENCES / Rats / Substrate Specificity / Protein Conformation / Amino Acid Sequence / Structure activity Relationship / Recombinant Proteins / Pulmonary Surfactant / Molecular Sequence Data

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REBASE: restriction enzymes and methyltransferases

Biological Sciences / Environmental Sciences / DNA methylation / Restriction-Modification Systems / Nucleic Acids / Enzyme / Substrate Specificity / Enzyme / Substrate Specificity

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Protein Farnesyltransferase and Protein Prenylation in Plasmodium falciparum

Enzyme Inhibitors / Biological Chemistry / Biological Sciences / Animals / Biological / Enzyme / CHEMICAL SCIENCES / Diterpenes / Substrate Specificity / Plasmodium falciparum / Antimalarials / Amino Acid Profile / Protozoan Proteins / Enzyme / CHEMICAL SCIENCES / Diterpenes / Substrate Specificity / Plasmodium falciparum / Antimalarials / Amino Acid Profile / Protozoan Proteins

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Biochemical characterization of recombinant serotonin N-acetyltransferase

Engineering / Biochemistry / Thermodynamics / Technology / Catalysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis

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One Polypeptide with Two Aminoacyl-tRNA Synthetase Activities

Genetics / Molecular Evolution / Science / Multidisciplinary / Protein synthesis / Escherichia coli / Proline / Enzyme / Protein Sequence Analysis / Substrate Specificity / Multienzyme complexes / Cysteine / Genome sequence / aminoacyl-tRNA synthetase / Escherichia coli / Proline / Enzyme / Protein Sequence Analysis / Substrate Specificity / Multienzyme complexes / Cysteine / Genome sequence / aminoacyl-tRNA synthetase

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Biochemical Characterization of an L-Xylulose Reductase from Neurospora crassa

Kinetics / Gene expression / Multidisciplinary / Neurospora crassa / Glucose Metabolism / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway / Xylitol / Escherichia coli / Applied Environmental Microbiology / Temperature / Enzyme / Molecular cloning / Substrate Specificity / Guinea Pig / Xylose Reductase / Heterologous Expression / Recombinant Proteins / Hydrogen-Ion Concentration / Pigeon / Pentose Phosphate Pathway

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Aspartic Proteinase in Dugesia tigrina (Girard) Planaria

Kinetics / Animals / High Pressure Liquid Chromatography / Protease Inhibitors / Substrate Specificity / Molecular weight / Hydrogen-Ion Concentration / Planarians / Molecular weight / Hydrogen-Ion Concentration / Planarians

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Detection of NADPH-diaphorase activity in Paramecium primaurelia

Microbiology / Medical Microbiology / Immunohistochemistry / Enzyme Inhibitors / Animals / Acetylcholine / Rat Brain / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Enzyme / European / Active site / Nitric Oxide Synthase / Substrate Specificity / Molecular Mass / NAD / Hydrogen-Ion Concentration / Paramecium / Cell Cell Interaction / Immunoblotting / Acetylcholine / Rat Brain / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Enzyme / European / Active site / Nitric Oxide Synthase / Substrate Specificity / Molecular Mass / NAD / Hydrogen-Ion Concentration / Paramecium / Cell Cell Interaction / Immunoblotting

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Human Hydroxysteroid Sulfotransferase SULT2B1: Two Enzymes Encoded by a Single Chromosome 19 Gene

Information Systems / Pharmacology / Genetics / Genomics / Confocal Microscopy / Gene expression / Functional Genomics / Cercopithecus aethiops / Pregnancy / Humans / Mutation / Placenta / Haplotypes / Pharmacogenetics / Female / Animals / Male / Homology / Alternative splicing / Prostate / Enzyme / African Americans / Molecular cloning / Trachea / Substrate Specificity / Isoenzymes / Linkage Disequilibrium / European Continental Ancestry Group / Dehydroepiandrosterone / Amino Acid Profile / Alternative Splicing / Amino Acid Sequence / Base Sequence / Genetic Mapping / Open Reading Frame / Recombinant Proteins / Small Intestine / Cysteine Proteinase Inhibitors / Molecular Sequence Data / Gene expression / Functional Genomics / Cercopithecus aethiops / Pregnancy / Humans / Mutation / Placenta / Haplotypes / Pharmacogenetics / Female / Animals / Male / Homology / Alternative splicing / Prostate / Enzyme / African Americans / Molecular cloning / Trachea / Substrate Specificity / Isoenzymes / Linkage Disequilibrium / European Continental Ancestry Group / Dehydroepiandrosterone / Amino Acid Profile / Alternative Splicing / Amino Acid Sequence / Base Sequence / Genetic Mapping / Open Reading Frame / Recombinant Proteins / Small Intestine / Cysteine Proteinase Inhibitors / Molecular Sequence Data

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Probing the Breadth of Macrolide Glycosyltransferases: In Vitro Remodeling of a Polyketide Antibiotic Creates Active Bacterial Uptake and Enhances Potency

The / Gram Positive Bacteria / Glycosylation / CHEMICAL SCIENCES / Anti-Bacterial Agents / Substrate Specificity / Microbial Sensitivity Tests / Erythromycin / Substrate Specificity / Microbial Sensitivity Tests / Erythromycin

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Mitochondrial targeted coenzyme Q, superoxide, and fuel selectivity in endothelial cells

Engineering / Physics / Chemistry / Biology / Mitochondria / Medicine / Multidisciplinary / Antioxidants / Glucose / Endothelial Cells / Reactive Oxygen Species / Animals / PLoS one / Cattle / Endothelial cell / Substrate Specificity / Oleic Acid / Aorta / Coenzyme Q / Superoxides / Medicine / Multidisciplinary / Antioxidants / Glucose / Endothelial Cells / Reactive Oxygen Species / Animals / PLoS one / Cattle / Endothelial cell / Substrate Specificity / Oleic Acid / Aorta / Coenzyme Q / Superoxides

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Suicide inactivation of peroxidase from Chamaerops excelsa palm tree leaves

Enzymology / Chromatography / Kinetics / Biotechnology / Protein Science / Enzymes / Peroxidase / Arecaceae / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Enzyme / Hydrogen Peroxide / Time Dependent / Solutions / Substrate Specificity / Biological Macromolecules / Hydrogen-Ion Concentration / PLANT PROTEINS / Oxidation-Reduction / Guaiacol / Biochemistry and cell biology / Plant Leaves / Enzymes / Peroxidase / Arecaceae / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Enzyme / Hydrogen Peroxide / Time Dependent / Solutions / Substrate Specificity / Biological Macromolecules / Hydrogen-Ion Concentration / PLANT PROTEINS / Oxidation-Reduction / Guaiacol / Biochemistry and cell biology / Plant Leaves

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Antifungal Tradecraft by Cholesterol Oxidase

Organic Chemistry / Catalysis / Statistical Analysis / Protein Engineering / High throughput screening / Cholesterol / Pseudomonas / Enzyme / Substrate Specificity / Antifungal Agents / Chemical Properties / Chemistry Biology / Ergosterol / Function Space / Enzymatic Activity / Biochemistry and cell biology / Cholesterol / Pseudomonas / Enzyme / Substrate Specificity / Antifungal Agents / Chemical Properties / Chemistry Biology / Ergosterol / Function Space / Enzymatic Activity / Biochemistry and cell biology

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Local RNA Target Structure Influences siRNA Efficacy: A Systematic Global Analysis

Thermodynamics / Molecular Biology / Computational Biology / Gene Silencing / Global Analysis / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus

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Local RNA Target Structure Influences siRNA Efficacy: A Systematic Global Analysis

Thermodynamics / Molecular Biology / Computational Biology / Gene Silencing / Global Analysis / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus / Genetic Engineering / Molecular / RNA interference / Cercopithecus aethiops / Cell line / Humans / RNAi / Animals / Drug Design / Free Energy / Rats / dsRNA / Substrate Specificity / Fluorescent Protein / Transfection / siRNA / RNA Structure / Base Sequence / TRP / UTR / GFP / Nucleic Acid Conformation / Biochemistry and cell biology / Enterovirus

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Substrate specificity of recombinant dengue 2 virus NS2BNS3 protease: Influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substrates

Biophysics / Kinetics / Fluorescent Dyes and Reagents / Fluorescence Resonance Energy Transfer / In Vitro / Identification / Substrate Specificity / Cleavage / Amino Acid Profile / Non-structural Measure / Salts / Recombinant Proteins / Hydrolysis / Hydrogen-Ion Concentration / Biochemistry and cell biology / Furin / Identification / Substrate Specificity / Cleavage / Amino Acid Profile / Non-structural Measure / Salts / Recombinant Proteins / Hydrolysis / Hydrogen-Ion Concentration / Biochemistry and cell biology / Furin

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